Fetal Bovine Goat Horse Serum

• Customization: Available
• Supply Type: OEM Service
• Usage Mode: For external use
• Type: Biological Diagnostic Reagents
• Application: for Diagnosis
• Form: Liquid
• Trademark: OEM
• Origin: China
• HS Code: 38220010
• Production Capacity: 500000 Pieces/Year

Product Description

Product Name:

Bovine Serum

Product Type :

Goat Serum

Code :

CSB-EL007403HU

Size :

96T,5×96T,10×96T

Uniprot NO. :

P25101

Abbreviation :

EDNRA

Alias :

ETA, ETAR, ETRA, G protein-coupled receptor|endothelin ET-A receptor|endothelin receptor subtype A|endothelin receptor, ET1-specific type|endothelin-1-specific receptor

Species :

Human

Sample Types :

serum, plasma, tissue homogenates

Detect Range :

47 pg/ml-3000 pg/ml

Sensitivity :

11.75 pg/ml

Assay Time :

1-5h

Sample Volume :

50-100ul

Detection Wavelengt :

450 nm

Availability :

7-14 working days

Research Area :

Cancer

Protocol :

 Instructions
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Target details :

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants.

HGNC :

3179

RGD :

2535

MGI :

105923

Precision :

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity :

To assess the linearity of the assay, samples were spiked with high concentrations of human EDNRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		103
		Range %		96-107
1:2		Average %		100
		Range %		91-104
1:4		Average %		88
		Range %		81-92
1:8		Average %		94
		Range %		84-98

Recovery :

The recovery of human EDNRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		91		84-96
EDTA plasma (n=4)		102		93-106

Typical Data :

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

pg/ml	OD1	OD2	Average	Corrected
3000	2.761	2.685	2.723	2.549
1500	2.318	2.452	2.385	2.211
750	1.845	1.989	1.917	1.743
375	1.245	1.223	1.234	1.060
187.5	0.773	0.743	0.758	0.584
94	0.570	0.556	0.563	0.389
47	0.384	0.374	0.379	0.205
0	0.172	0.176	0.174